Carbapenem antibiotic stress increases bla_{KPC -2} gene relative copy number and bacterial resistance levels of Klebsiella pneumoniae.

ABSTRACT:

Background

The clinical isolation rates of carbapenem-resistant Klebsiella pneumoniae (CR-KP) continue to increase. In China, clinical CR-KP isolates are mainly attributed to the bla_KPC-2 gene carried on plasmids, and the bla_KPC-2 copy number correlates with the expression of KPC enzymes, which can cause elevated carbapenem MICs.

Methods

Thirty-seven CR-KP isolates were collected at the Second People's Hospital of Lianyungang City between January 2020 and March 2021, with no duplicate isolates, and were screened for the bla_KPC-2 gene with PCR. Analysis of current CRKP resistance to clinically relevant antimicrobials using the bioMérieux VITEK^® 2 bacterial identification card. The multilocus sequence types of the strains were confir

SUBMITTER: Chen H 

PROVIDER: S-EPMC9280016 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature