Effect of high glucose on glycosaminoglycans in cultured retinal endothelial cells and rat retina.

ABSTRACT:

Introduction

The endothelial glycocalyx regulates vascular permeability, inflammation, and coagulation, and acts as a mechanosensor. The loss of glycocalyx can cause endothelial injury and contribute to several microvascular complications and, therefore, may promote diabetic retinopathy. Studies have shown a partial loss of retinal glycocalyx in diabetes, but with few molecular details of the changes in glycosaminoglycan (GAG) composition. Therefore, the purpose of our study was to investigate the effect of hyperglycemia on GAGs of the retinal endothelial glycocalyx.

Methods

GAGs were isolated from rat retinal microvascular endothelial cells (RRMECs), media, and retinas, followed by liquid chromatography-mass spectrometry assays. Quantitative real-time polymerase chain react

SUBMITTER: Kaur G 

PROVIDER: S-EPMC9280546 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature