Electrophoresis-Mediated Characterization of Full and Empty Adeno-Associated Virus Capsids.

ABSTRACT: Adeno-associated virus (AAV) has shown great potential in gene therapy due to its low immunogenicity, lack of pathogenicity to humans, and ability to provide long-term gene expression in vivo. However, there is currently a need for fast, high-throughput characterization systems that require low volumes for the determination of its sample composition in terms of full and empty capsids since empty capsids are a natural byproduct of AAV synthesis. To address this need, the following study proposes a high-throughput electrophoresis-mediated microfluidics approach that is independent of sample input concentration to estimate the composition of a given sample by combining its protein and ssDNA information relative to a standard. Using this novel approach, we were able to estimate the perc

SUBMITTER: Coll De Pena A 

PROVIDER: S-EPMC9280930 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature