Development of a Clinically Relevant Reporter for Chimeric Antigen Receptor T-cell Expansion, Trafficking, and Toxicity.

ABSTRACT: Although chimeric antigen receptor T (CART)-cell therapy has been successful in treating certain hematologic malignancies, wider adoption of CART-cell therapy is limited because of minimal activity in solid tumors and development of life-threatening toxicities, including cytokine release syndrome (CRS). There is a lack of a robust, clinically relevant imaging platform to monitor in vivo expansion and trafficking to tumor sites. To address this, we utilized the sodium iodide symporter (NIS) as a platform to image and track CART cells. We engineered CD19-directed and B-cell maturation antigen (BCMA)-directed CART cells to express NIS (NIS⁺CART19 and NIS⁺BCMA-CART, respectively) and tested the sensitivity of ¹⁸F-TFB-PET to detect trafficking and expansi

SUBMITTER: Sakemura R 

PROVIDER: S-EPMC9280998 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature