DCSr-NL: A Novel Method to Semiquantitatively Probe the Growth Rate of Nacre.

ABSTRACT: Matrix proteins play critical roles in regulating the prismatic and nacreous layer formation in the shell. However, due to the dearth of in vivo experiments, their specific roles during shell formation are still unclear. In this study, a new method to detect the content of Sr in the nacreous layer (DCSr-NL), which can semiquantitatively measure the nacreous growth rate, has been proposed. In vitro experiments show that during in vitro crystallization, the Sr element can replace Ca partially, resulting in isomorphism. In vivo experiments show that the best labeling conditions are when the Sr/Ca in seawater is 0.3, at 24 °C, and at 4 days of culture. Although a surface morphological difference in the inner layer of nacre is seldom detected by scanning electron mic

SUBMITTER: Yi L 

PROVIDER: S-EPMC9281326 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature