The microtubule plus-end tracking protein Bik1 is required for chromosome congression.

ABSTRACT: During mitosis, sister chromatids congress on both sides of the spindle equator to facilitate the correct partitioning of the genomic material. Chromosome congression requires a finely tuned control of microtubule dynamics by the kinesin motor proteins. In Saccharomyces cerevisiae, the kinesin proteins Cin8, Kip1, and Kip3 have a pivotal role in chromosome congression. It has been hypothesized that additional proteins that modulate microtubule dynamics are involved. Here, we show that the microtubule plus-end tracking protein Bik1-the budding yeast ortholog of CLIP-170-is essential for chromosome congression. We find that nuclear Bik1 localizes to the kinetochores in a cell cycle-dependent manner. Disrupting the nuclear pool of Bik1 with a nuclear export signal (Bik1-NES) leads to s

SUBMITTER: Julner A 

PROVIDER: S-EPMC9282014 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature