Methods for Detecting PER2:LUCIFERASE Bioluminescence Rhythms in Freely Moving Mice.

ABSTRACT: Circadian rhythms are driven by daily oscillations of gene expression. An important tool for studying cellular and tissue circadian rhythms is the use of a gene reporter, such as bioluminescence from the reporter gene luciferase controlled by a rhythmically expressed gene of interest. Here we describe methods that allow measurement of circadian bioluminescence from a freely moving mouse housed in a standard cage. Using a LumiCycle In Vivo (Actimetrics), we determined conditions that allow detection of circadian rhythms of bioluminescence from the PER2 reporter, PER2::LUC, in freely behaving mice. The LumiCycle In Vivo applies a background subtraction that corrects for effects of room temperature on photomultiplier tube (PMT) output. We tested delivery of d-luciferin via a subcutaneous mini

SUBMITTER: Martin-Burgos B 

PROVIDER: S-EPMC9282071 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature