Comprehensive analysis of molecular pathways and key genes involved in lumbar disc herniation.

ABSTRACT:

Abstract

Based on the Thompson classification of intervertebral discs (IVDs), we systematically analyzed gene expression differences between severely degenerated and mildly degenerated IVDs and explored the underlying molecular mechanisms using bioinformatics methods and multichip integration. We used multiomics analysis, includes mRNA microarray and methylation chips, to explore the genetic network and mechanisms of lumbar disc herniation (LDH). Subsequently, the Combat function of the R language SVA package was applied to eliminate heterogeneity between the gene expression data. And the protein-protein interaction (PPI) network, gene ontology (GO), and molecular pathways were used to constructs the mechanisms network. Consequently, we obtained 149 differentially expressed genes.

SUBMITTER: Liu Q 

PROVIDER: S-EPMC9282072 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature