Factors Compromising Glucuronidase Performance in Urine Drug Testing Potentially Resulting in False Negatives.

ABSTRACT: Next generation β-glucuronidases can effectively cleave glucuronides in urine at room temperature. However, during the discovery studies, additional challenges were identified for urine drug testing across biologically relevant pH extremes and patient urine specimens. Different enzymes were evaluated across clinical urine specimens and commercially available urine control matrices. Each enzyme shows distinct substrate preferences, pH optima, and variability across clinical specimens. These results demonstrate how reliance on a single glucuronidated substrate as the internal hydrolysis control cannot ensure performance across a broader panel of analytes. Moreover, sample specific urine properties compromise β-glucuronidases to varying levels, more pronounced for some enzymes, and thereby lo

SUBMITTER: Lee LA 

PROVIDER: S-EPMC9282255 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature