Green fluorescent cAMP indicator of high speed and specificity suitable for neuronal live-cell imaging.

ABSTRACT: Cyclic adenosine monophosphate (cAMP) is a canonical intracellular messenger playing diverse roles in cell functions. In neurons, cAMP promotes axonal growth during early development, and mediates sensory transduction and synaptic plasticity after maturation. The molecular cascades of cAMP are well documented, but its spatiotemporal profiles associated with neuronal functions remain hidden. Hence, we developed a genetically encoded cAMP indicator based on a bacterial cAMP-binding protein. This indicator "gCarvi" monitors [cAMP]_i at 0.2 to 20 µM with a subsecond time resolution and a high specificity over cyclic guanosine monophosphate (cGMP). gCarvi can be converted to a ratiometric probe for [cAMP]_i quantification and its expression can be specifically targeted to va

SUBMITTER: Kawata S 

PROVIDER: S-EPMC9282276 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature