Voltage-gated sodium channel scn8a is required for innervation and regeneration of amputated adult zebrafish fins.

ABSTRACT: Teleost fishes and urodele amphibians can regenerate amputated appendages, whereas this ability is restricted to digit tips in adult mammals. One key component of appendage regeneration is reinnervation of the wound area. However, how innervation is regulated in injured appendages of adult vertebrates has seen limited research attention. From a forward genetics screen for temperature-sensitive defects in zebrafish fin regeneration, we identified a mutation that disrupted regeneration while also inducing paralysis at the restrictive temperature. Genetic mapping and complementation tests identify a mutation in the major neuronal voltage-gated sodium channel (VGSC) gene scn8ab. Conditional disruption of scn8ab impairs early regenerative events, including blastema formation, but

SUBMITTER: Osorio-Mendez D 

PROVIDER: S-EPMC9282381 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature