Antiprion systems in yeast cooperate to cure or prevent the generation of nearly all [PSI⁺] and [URE3] prions.

ABSTRACT: [PSI⁺] and [URE3] are prions of Saccharomyces cerevisiae based on amyloids of Sup35p and Ure2p, respectively. In normal cells, antiprion systems block prion formation, cure many prions that arise, prevent infection by prions, and prevent toxicity of those prions that escape the other systems. The upf1Δ, ssz1Δ, and hsp104^T160M single mutants each develop [PSI⁺] at 10- to 15-fold, but the triple mutant spontaneously generates [PSI⁺] at up to ∼5,000-fold the wild-type rate. Most such [PSI⁺] variants are cured by restoration of any one of the three defective antiprion systems, defining a previously unknown type of [PSI⁺] variant and proving that these three antiprion

SUBMITTER: Son M 

PROVIDER: S-EPMC9282430 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature