Development and validation of an LC-MS/MS method for determination of B vitamins and some its derivatives in whole blood.

ABSTRACT: Obligate symbiotic bacteria associated with the insects feeding exclusively on vertebrate blood are supposed to complement B vitamins presumably lacking in their diet. Recent genomic analyses revealed considerable differences in biosynthetic capacities across different symbionts, suggesting that levels of B vitamins may vary across different vertebrate hosts. However, a rigorous determination of B vitamins content in blood of various vertebrates has not yet been approached. A reliable analytical method focused on B vitamin complex in blood can provide valuable informative background and understanding of general principles of insect symbiosis. In this work, a chromatographic separation of eight B vitamins (thiamine, riboflavin, niacin, pantothenic acid, pyridoxine, biotin, folic acid, and c

SUBMITTER: Kahoun D 

PROVIDER: S-EPMC9282541 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature