Microphysiological system for studying contractile differences in young, active, and old, sedentary adult derived skeletal muscle cells.

ABSTRACT: Microphysiological systems (MPS), also referred to as tissue chips, incorporating 3D skeletal myobundles are a novel approach for physiological and pharmacological studies to uncover new medical treatments for sarcopenia. We characterize a MPS in which engineered skeletal muscle myobundles derived from donor-specific satellite cells that model aged phenotypes are encapsulated in a perfused tissue chip platform containing platinum electrodes. Our myobundles were derived from CD56⁺ myogenic cells obtained via percutaneous biopsy of the vastus lateralis from adults phenotyped by age and physical activity. Following 17 days differentiation including 5 days of a 3 V, 2 Hz electrical stimulation regime, the myobundles exhibited fused myotube alignment and upregulation of myogenic, myo

SUBMITTER: Giza S 

PROVIDER: S-EPMC9282836 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature