IgM-associated gut bacteria in obesity and type 2 diabetes in C57BL/6 mice and humans.

ABSTRACT:

Aims/hypothesis

IgM is the primary antibody produced by B cells and we hypothesise that IgM antibodies to gut microbiota may play a role in immunometabolism in obesity and type 2 diabetes. To test our hypothesis, we used B6 mice deficient in activation-induced cytidine deaminase (Aid^-/- [also known as Aicda^-/-]) which secrete only IgM antibodies, and human faecal samples.

Methods

We studied the immunometabolic effects and gut microbial changes in high-fat-diet-induced obesity (HFDIO) in Aid^-/- B6 mice compared with wild-type mice. To determine similarities between mice and humans, human stool samples were collected from children and adolescents who were obese with normal glucose tolerance (NGT), obese with glucose intolerance (IGT), or obese

SUBMITTER: Pearson JA 

PROVIDER: S-EPMC9283171 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature