Multi-omics study identifies novel signatures of DNA/RNA, amino acid, peptide, and lipid metabolism by simulated diabetes on coronary endothelial cells.

ABSTRACT: Coronary artery endothelial cells (CAEC) exert an important role in the development of cardiovascular disease. Dysfunction of CAEC is associated with cardiovascular disease in subjects with type 2 diabetes mellitus (T2DM). However, comprehensive studies of the effects that a diabetic environment exerts on this cellular type are scarce. The present study characterized the molecular perturbations occurring on cultured bovine CAEC subjected to a prolonged diabetic environment (high glucose and high insulin). Changes at the metabolite and peptide level were assessed by Liquid Chromatography-Mass Spectrometry (LC-MS²) and chemoinformatics. The results were integrated with published LC-MS²-based quantitative proteomics on the same in vitro model. Our findings were consisten

SUBMITTER: Moreno-Ulloa A 

PROVIDER: S-EPMC9283518 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature