A computational approach to quantifying miscounting of radiation-induced double-strand break immunofluorescent foci.

ABSTRACT: Immunofluorescent tagging of DNA double-strand break (DSB) markers, such as γ-H2AX and other DSB repair proteins, are powerful tools in understanding biological consequences following irradiation. However, whilst the technique is widespread, there are many uncertainties related to its ability to resolve and reliably deduce the number of foci when counting using microscopy. We present a new tool for simulating radiation-induced foci in order to evaluate microscope performance within in silico immunofluorescent images. Simulations of the DSB distributions were generated using Monte Carlo track-structure simulation. For each DSB distribution, a corresponding DNA repair process was modelled and the un-repaired DSBs were recorded at several time points. Corresponding microscopy images for both

SUBMITTER: Ingram SP 

PROVIDER: S-EPMC9283546 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature