Auxotrophic and prototrophic conditional genetic networks reveal the rewiring of transcription factors in Escherichia coli.

ABSTRACT: Bacterial transcription factors (TFs) are widely studied in Escherichia coli. Yet it remains unclear how individual genes in the underlying pathways of TF machinery operate together during environmental challenge. Here, we address this by applying an unbiased, quantitative synthetic genetic interaction (GI) approach to measure pairwise GIs among all TF genes in E. coli under auxotrophic (rich medium) and prototrophic (minimal medium) static growth conditions. The resulting static and differential GI networks reveal condition-dependent GIs, widespread changes among TF genes in metabolism, and new roles for uncharacterized TFs (yjdC, yneJ, ydiP) as regulators of cell division, putrescine utilization pathway, and cold shock adaptation. Pan-bacterial conservation suggests TF genes with GIs are

SUBMITTER: Gagarinova A 

PROVIDER: S-EPMC9283627 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature