Comprehensive metabolome analysis of intracellular metabolites in cultured cells.
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ABSTRACT:
SUBMITTER: Kudo R
PROVIDER: S-EPMC9283946 | biostudies-literature | 2022 Sep
REPOSITORIES: biostudies-literature
Publications
Comprehensive metabolome analysis of intracellular metabolites in cultured cells.
Kudo Ryuhei R Igarashi Kaori K Soga Tomoyoshi T Ishikawa Takamasa T Saito Yasuhiro Y
STAR protocols 20220711 3
Capillary electrophoresis mass spectrometry (CE-MS) can measure the intracellular amount of highly polar and charged metabolites; liquid chromatography mass spectrometry (LC-MS) can quantify hydrophobic metabolites. A comprehensive metabolome analysis requires independent sample preparation for LC-MS and CE-MS. Here, we present a protocol to prepare for sequentially analyzing the metabolites from one sample. Here we describe the steps for breast cancer cell lines, MCF-7 cells, but the protocol c ...[more]
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