Procoagulant platelet sentinels prevent inflammatory bleeding through GPIIBIIIA and GPVI.

ABSTRACT: Impairment of vascular integrity is a hallmark of inflammatory diseases. We recently reported that single immune-responsive platelets migrate and reposition themselves to sites of vascular injury to prevent bleeding. However, it remains unclear how single platelets preserve vascular integrity once encountering endothelial breaches. Here we demonstrate by intravital microscopy combined with genetic mouse models that procoagulant activation (PA) of single platelets and subsequent recruitment of the coagulation cascade are crucial for the prevention of inflammatory bleeding. Using a novel lactadherin-based compound, we detect phosphatidylserine (PS)-positive procoagulant platelets in the inflamed vasculature. We identify exposed collagen as the central trigger arresting platelets and initiati

SUBMITTER: Kaiser R 

PROVIDER: S-EPMC9283965 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature