Altered Regulation of mRNA and miRNA Expression in Epithelial and Stromal Tissue of Keratoconus Corneas.

ABSTRACT:

Purpose

Evaluation of mRNA and microRNA (miRNA) expression in epithelium and stroma of patients with keratoconus.

Methods

The epithelium and stroma of eight corneas of eight patients with keratoconus and eight corneas of eight non-keratoconus healthy controls were studied separately. RNA was extracted, and mRNA and miRNA analyses were performed using microarrays. Differentially expressed mRNAs and miRNAs in epithelial and stromal keratoconus samples compared to healthy controls were identified. Selected genes and miRNAs were further validated using RT-qPCR.

Results

We discovered 170 epithelial and 1498 stromal deregulated protein-coding mRNAs in KC samples. In addition, in epithelial samples 180 miRNAs and in stromal samples 379 miRNAs were significantly deregulated m

SUBMITTER: Stachon T 

PROVIDER: S-EPMC9284461 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature