Differentiated kidney tubular cell-derived extracellular vesicles enhance maturation of tubuloids.

ABSTRACT: The prevalence of end-stage kidney disease (ESKD) is rapidly increasing with the need for regenerative therapies. Adult stem cell derived kidney tubuloids have the potential to functionally mimic the adult kidney tubule, but still lack the expression of important transport proteins needed for waste removal. Here, we investigated the potential of extracellular vesicles (EVs) obtained from matured kidney tubular epithelial cells to modulate in vitro tubuloids functional maturation. We focused on organic anion transporter 1 (OAT1), one of the most important proteins involved in endogenous waste excretion. First, we show that EVs from engineered proximal tubule cells increased the expression of several transcription factors and epithelial transporters, resulting in improved OAT1 transport capa

SUBMITTER: Lindoso RS 

PROVIDER: S-EPMC9284832 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature