Highly sensitive quantification of pemetrexed in human plasma using UPLC-MS/MS to support microdosing studies.

ABSTRACT: Pemetrexed is an antifolate drug approved for the treatment of non-small-cell lung cancer and mesothelioma. Assessing pemetrexed pharmacokinetics after administration of a microdose (100 μg) may facilitate drug-drug interaction and dose individualization studies with cytotoxic drugs, without causing harm to patients. Therefore, a highly sensitive bioanalytical assay is required. A reversed-phase ultra-high performance liquid chromatography method was developed to determine pemetrexed concentrations in human ethylenediaminetetraacetic acid-plasma after microdosing. [¹³ C₅ ]-Pemetrexed was used as the internal standard. The sample preparation involved solid-phase extraction from plasma. Detection was performed using MS/MS in a total run time of 9.5 min. The assay was va

SUBMITTER: van Ewijk-Beneken Kolmer EWJ 

PROVIDER: S-EPMC9285051 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature