Cyclocytidine hydrochloride inhibits the synthesis of relaxed circular DNA of hepatitis B virus.

ABSTRACT:

Background

Cyclocytidine hydrochloride (HCl) has been reported to inhibit DNA synthesis by affecting DNA polymerase. Here, we tested the antiviral effect of cyclocytidine on hepatitis B virus (HBV) DNA synthesis, which is reliant on DNA polymerase activity.

Materials and methods

Cyclocytidine HCl was treated to HBV-producing HepAD38 cells or added to an endogenous polymerase reaction, and HBV DNA was detected by Southern blot.

Results

Treatment of 20 µM cyclocytidine HCl significantly decreased the production of relaxed circular (rc) DNA in HepAD38 cells and block rcDNA synthesis in endogenous polymerase reaction (EPR), a cell free assay, possibly by inhibiting the HBV DNA polymerase activity.

Conclusion

Cyclocytidine HCl could inhibit the synthesis of HBV rcDN

SUBMITTER: Wang X 

PROVIDER: S-EPMC9285472 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature