Characterization of human tear proteome reveals differentially abundance proteins in thyroid-associated ophthalmopathy.

ABSTRACT:

Background

Thyroid-associated ophthalmopathy (TAO) is a common orbital inflammatory disease, but the abnormal expression of proteins in tears of TAO patients has not been systematically studied. The purpose of this study is to compare and analyze the total tear protein profile of TAO patients and to provide protein cues for TAO pathogenesis.

Methods

Tear samples were isolated from 30 TAO patients with obvious ocular surface damage and 30 healthy control subjects. Tear samples from 30 individuals were mixed and divided into three sample pools. Easy nano-scale LC-MS/MS based on labeling-free quantitative technology was utilized to profile tear proteome.

Results

Here, electrospray ionization mass spectra and SDS-PAGE results confirmed the good parallelisms among samples.

SUBMITTER: Zhou X 

PROVIDER: S-EPMC9285480 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature