An optimized MALDI MSI protocol for spatial detection of tryptic peptides in fresh frozen prostate tissue.

ABSTRACT: MALDI MS imaging (MSI) is a powerful analytical tool for spatial peptide detection in heterogeneous tissues. Proper sample preparation is crucial to achieve high quality, reproducible measurements. Here we developed an optimized protocol for spatially resolved proteolytic peptide detection with MALDI time-of-flight MSI of fresh frozen prostate tissue sections. The parameters tested included four different tissue washes, four methods of protein denaturation, four methods of trypsin digestion (different trypsin densities, sprayers, and incubation times), and five matrix deposition methods (different sprayers, settings, and matrix concentrations). Evaluation criteria were the number of detected and excluded peaks, percentage of high mass peaks, signal-to-noise ratio, spatial localization, and

SUBMITTER: Hoiem TS 

PROVIDER: S-EPMC9285595 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature