Identification of compendial nonionic detergents for the replacement of Triton X-100 in bioprocessing.

ABSTRACT: We have systematically investigated six compendial nonionic detergents as potential replacements for Triton ×-100 in bioprocessing applications. Use of compendial raw materials in cGMP bioprocessing is advantageous for a variety of reasons including material specifications developed to meet stringent pharmaceutical product quality requirements, regulatory familiarity and comfort, and availability from vendors experienced supplying the biopharmaceutical industry. We first examine material properties of the detergents themselves including melting point and viscosity. Process performance and product contact in real-world bioprocess applications are then investigated. Lastly, we test the detergents in virus inactivation (VI) experiments with recombinant proteins and adeno-associated virus. Two

SUBMITTER: Hunter AK 

PROVIDER: S-EPMC9285696 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature