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Optimization of integrated chromatography sequences for purification of biopharmaceuticals.

ABSTRACT: With continued development of integrated and continuous downstream purification processes, tuning and optimization become increasingly complicated with additional parameters and codependent variables over the sequence. This article offers a novel perspective of nonlinear optimization of integrated sequences with regard to individual column sizes, flow rates, and scheduling. The problem setup itself is a versatile tool to be used in downstream design which is demonstrated in two case studies: a four-column integrated sequence and a continuously loaded twin-capture setup with five columns.

SUBMITTER: Lofgren A

PROVIDER: S-EPMC9285797 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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Optimization of integrated chromatography sequences for purification of biopharmaceuticals.

Löfgren Anton A Yamanee-Nolin Mikael M Tallvod Simon S Fons Joaquín G JG Andersson Niklas N Nilsson Bernt B

Biotechnology progress 20190701 6

With continued development of integrated and continuous downstream purification processes, tuning and optimization become increasingly complicated with additional parameters and codependent variables over the sequence. This article offers a novel perspective of nonlinear optimization of integrated sequences with regard to individual column sizes, flow rates, and scheduling. The problem setup itself is a versatile tool to be used in downstream design which is demonstrated in two case studies: a f ...[more]

PMID: 31207182

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