Electronic cigarette liquids impair metabolic cooperation and alter proteomic profiles in V79 cells.

ABSTRACT:

Background

Although still considered a safer alternative to classical cigarettes, growing body of work points to harmful effects of electronic cigarettes (e-cigarettes) affecting a range of cellular processes. The biological effect of e-cigarettes needs to be investigated in more detail considering their widespread use.

Methods

In this study, we treated V79 lung fibroblasts with sub-cytotoxic concentration of e-cigarette liquids, with and without nicotine. Mutagenicity was evaluated by HPRT assay, genotoxicity by comet assay and the effect on cellular communication by metabolic cooperation assay. Additionally, comprehensive proteome analysis was performed via high resolution, parallel accumulation serial fragmentation-PASEF mass spectrometry.

Results

E-cigarette liqui

SUBMITTER: Trifunovic S 

PROVIDER: S-EPMC9285873 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature