Establishment of a reference panel of Helicobacter pylori strains for antimicrobial susceptibility testing.

ABSTRACT:

Background

Eradication treatment for Helicobacter pylori gastritis is covered by national health insurance since 2013 in Japan. However, eradication failure due to the increase of antimicrobial resistance has become a serious problem. The present study aims to establish a reference panel of Japanese H. pylori strains for antimicrobial susceptibility testing.

Method

A total of 28 strains were collected from 4 medical facilities in Japan. Antimicrobial susceptibility tests (ASTs) to clarithromycin (CLR), amoxicillin (AMX), and metronidazole (MNZ), were used to select standard reference strains. Complete genome sequences were also determined.

Results

Three H. pylori strains (JSHR3, JSHR6 and JSHR31) were selected as standard reference strains by the Japanese Society for

SUBMITTER: Yokota K 

PROVIDER: S-EPMC9286379 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature