Functional genomic analysis of epithelioid sarcoma reveals distinct proximal and distal subtype biology.

ABSTRACT:

Background

Metastatic epithelioid sarcoma (EPS) remains a largely unmet clinical need in children, adolescents and young adults despite the advent of EZH2 inhibitor tazemetostat.

Methods

In order to realise consistently effective drug therapies, a functional genomics approach was used to identify key signalling pathway vulnerabilities in a spectrum of EPS patient samples. EPS biopsies/surgical resections and cell lines were studied by next-generation DNA exome and RNA deep sequencing, then EPS cell cultures were tested against a panel of chemical probes to discover signalling pathway targets with the most significant contributions to EPS tumour cell maintenance.

Results

Other biologically inspired functional interrogations of EPS cultures using gene knockdown or chemi

SUBMITTER: Rasmussen SV 

PROVIDER: S-EPMC9286527 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature