Multiplex analysis of mass imaging data: Application to the pathology of experimental myocardial infarction.

ABSTRACT:

Aim

Imaging mass cytometry (IMC) affords simultaneous immune-labelling/imaging of multiple antigens in the same tissue. Methods utilizing multiplex data beyond co-registration are lacking. This study developed and applied an innovative spatial analysis workflow for multiplex imaging data to IMC data determined from cardiac tissues and revealed the mechanism(s) of neutrophil-mediated post-myocardial-infarction damage.

Methods

IMC produced multiplex images with various redox/inflammatory markers. The cardiac peri-infarct zone (PIZ) was determined to be up to 240 µm from the infarct border based on the presence of neutrophils. The tissue region beyond the infarct was defined as the remote area (RA). ImageJ was used to quantify the immunoreactivity. Functional assessments includ

SUBMITTER: Shi H 

PROVIDER: S-EPMC9286669 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature