Nuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus.

ABSTRACT: Retroviral infection involves the reverse transcription of the viral RNA genome into DNA, which is subsequently integrated into the host cell genome. Human immunodeficiency virus type 1 (HIV-1) and other lentiviruses mediate the infection of non-dividing cells through the ability of the capsid protein¹ to engage the cellular nuclear import pathways of the target cell and mediate their nuclear translocation through components of the nuclear pore complex^2-4. Although recent studies have observed the presence of the capsid protein in the nucleus during infection^5-8, reverse transcription and disassembly of the viral core have conventionally been considered to be cytoplasmic events. Here, we use an inducible nuclear pore complex blockade to monitor the kinetics

SUBMITTER: Dharan A 

PROVIDER: S-EPMC9286700 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature