"Time window" effect of Yoda1-evoked Piezo1 channel activity during mouse skeletal muscle differentiation.

ABSTRACT:

Aim

Mechanosensitive Piezo1 ion channels emerged recently as important contributors to various vital functions including modulation of the blood supply to skeletal muscles. The specific Piezo1 channel agonist Yoda1 was shown to regulate the tone of blood vessels similarly to physical exercise. However, the direct role of Piezo1 channels in muscle function has been little studied so far. We therefore investigated the action of Yoda1 on the functional state of skeletal muscle precursors (satellite cells and myotubes) and on adult muscle fibres.

Methods

Immunostaining, electrophysiological intracellular recordings and Ca²⁺ imaging experiments were performed to localize and assess the effect of the chemical activation of Piezo1 channels with Yoda1, on myogenic precurs

SUBMITTER: Bosutti A 

PROVIDER: S-EPMC9286833 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature