Evaluation of acquisition modes for semi-quantitative analysis by targeted and untargeted mass spectrometry.

ABSTRACT:

Rationale

Analyte quantitation by mass spectrometry underpins a diverse range of scientific endeavors. The fast-growing field of mass spectrometer development has resulted in several targeted and untargeted acquisition modes suitable for these applications. By characterizing the acquisition methods available on an ion mobility (IM)-enabled orthogonal acceleration time-of-flight (oa-ToF) instrument, the optimum modes for analyte semi-quantitation can be deduced.

Methods

Serial dilutions of commercial metabolite, peptide, or cross-linked peptide analytes were prepared in matrices of human urine or Escherichia coli digest. Each analyte dilution was introduced into an IM separation-enabled oa-ToF mass spectrometer by reversed-phase liquid chromatography and electrospray ionizati

SUBMITTER: Britt HM 

PROVIDER: S-EPMC9287043 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature