Transcription-associated DNA DSBs activate p53 during hiPSC-based neurogenesis.

ABSTRACT: Neurons are overproduced during cerebral cortical development. Neural progenitor cells (NPCs) divide rapidly and incur frequent DNA double-strand breaks (DSBs) throughout cortical neurogenesis. Although half of the neurons born during neurodevelopment die, many neurons with inaccurate DNA repair survive leading to brain somatic mosaicism. Recurrent DNA DSBs during neurodevelopment are associated with both gene expression level and gene length. We used imaging flow cytometry and a genome-wide DNA DSB capture approach to quantify and map DNA DSBs during human induced pluripotent stem cell (hiPSC)-based neurogenesis. Reduced p53 signaling was brought about by knockdown (p53^KD); p53^KD led to elevated DNA DSB burden in neurons that was associated with gene expression level

SUBMITTER: Michel N 

PROVIDER: S-EPMC9287420 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature