Building programmable multicompartment artificial cells incorporating remotely activated protein channels using microfluidics and acoustic levitation.

ABSTRACT: Intracellular compartments are functional units that support the metabolism within living cells, through spatiotemporal regulation of chemical reactions and biological processes. Consequently, as a step forward in the bottom-up creation of artificial cells, building analogous intracellular architectures is essential for the expansion of cell-mimicking functionality. Herein, we report the development of a droplet laboratory platform to engineer complex emulsion-based, multicompartment artificial cells, using microfluidics and acoustic levitation. Such levitated models provide free-standing, dynamic, definable droplet networks for the compartmentalisation of chemical species. Equally, they can be remotely operated with pneumatic, heating, and magnetic elements for post-processing, including

SUBMITTER: Li J 

PROVIDER: S-EPMC9287423 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature