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Assessing high-yielding cowpea varieties for bacterial blight resistance in Bangladesh: A step towards an environment-friendly and sustainable solution.

ABSTRACT: Cowpea is well-known worldwide for its high protein content, versatile use, and adaptability. However, it is devastatingly affected by bacterial blight disease caused by Xanthomonas axonopodis pv. vignicola (Xav). The present study was designed to assess ten high-yielding cowpea varieties for bacterial blight resistance in two contrasting cropping seasons in Bangladesh. The varieties were evaluated using seed and stem inoculation with Xav bacteria, followed by phenotypic and molecular characterisation. The varieties were morphologically assessed using nine disease-related qualitative and quantitative traits, and genetic variations were investigated through nine SSR markers. Disease development varied significantly (P = 0.05) among the varieties. Substantially higher disease incidence was observed in the Kharif season compared to the Rabi season. Felon local, Dark Green-28, and Dark Green-1028 varieties were resistant in both seasons. On the other hand, BARI Felon-1 was highly susceptible to susceptible in both seasons as infections were over 50%. Moreover, plant height, leaf area, branch number, and leaf number significantly differed among the varieties. Besides, in the molecular study, polymorphism information content and Nei's gene diversity were detected as 0.3658 and 0.4089, respectively. Kegornatki showed the highest genetic variation vs Dark Green-1028. The UPGMA dendrogram segregated the ten cowpea varieties into two main clusters. This study revealed that three high-yielding varieties, viz., Dark Green-28, Dark green 1028, and Felon local, were resistant to bacterial blight and showed better performance in morpho-molecular characterisation. Therefore, these varieties can be integrated into future cowpea breeding programmes to develop cultivars that can control the high pressures of Xav.

SUBMITTER: Saha S

PROVIDER: S-EPMC9287560 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Assessing high-yielding cowpea varieties for bacterial blight resistance in Bangladesh: A step towards an environment-friendly and sustainable solution.

Saha Sumitra S Romi Ismat Jahan IJ Khatun Fahmida F Saha Biplob Kumar BK Haque Muhammad Shahidul MS Saha Nihar Ranjan NR

Saudi journal of biological sciences 20220703 8

Cowpea is well-known worldwide for its high protein content, versatile use, and adaptability. However, it is devastatingly affected by bacterial blight disease caused by Xanthomonas axonopodis pv. vignicola (Xav). The present study was designed to assess ten high-yielding cowpea varieties for bacterial blight resistance in two contrasting cropping seasons in Bangladesh. The varieties were evaluated using seed and stem inoculation with Xav bacteria, followed by phenotypic and ...[more]

PMID: 35855768

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Project description:Cowpea, Vigna unguiculata (L.) Walp. is an important grain legume grown in the dry agro-ecologies of the tropics with considerably low yield due to lack of improved varieties, aggravated by prevalent narrow genetic base. Thus, induced mutagenesis was employed using sodium azide and gamma rays to increase genetic variability in cowpea genotypes that resulted in isolation of eleven high yielding mutant lines at the M4 generation from the genetic background of cowpea varieties Gomati VU-89 and Pusa-578. In order to analyze the induced genetic divergence among the mutant lines and parent genotypes, biochemical and molecular characterization was carried out with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), simple sequence repeat (SSR) and CAAT box derived polymorphism (CBDP) markers. Activity of nitrate reductase (NR) and content of chlorophyll, carotenoid, protein and mineral were found to be significantly high in the selected mutant lines compared to their respective parent genotypes. SDS-PAGE profile of seed proteins generated 54 and 28 polymorphic bands and a total polymorphism of 62.06 and 41.17% in Gomati VU-89 and Pusa-578, respectively. SSR primers amplified a total of 16 and 24 polymorphic bands with an average polymorphism of 20.69 and 50.74% in Gomati VU-89 and Pusa-578, respectively. CBDP markers, used for the first time in mutagenized population, generated 175 bands with 77 bands being polymorphic in Gomati VU-89 and 121 bands with 59 bands being polymorphic in Pusa-578. Physiological, biochemical and molecular profiling of the selected promising mutants lines showed that Gomati VU-89-G and Pusa-578-C are genetically most diverged high yielding genotypes with significant increase in protein and micronutrient content, therefore, could be recommended for further research considerations. Thus, the favorable combination of genes induced in the novel cowpea mutants selected through the present study are valuable to correspond farmers requirements for new improved cultivars (direct or hybrids).

Dataset Information

Assessing high-yielding cowpea varieties for bacterial blight resistance in Bangladesh: A step towards an environment-friendly and sustainable solution.

Publications

Assessing high-yielding cowpea varieties for bacterial blight resistance in Bangladesh: A step towards an environment-friendly and sustainable solution.

Similar Datasets

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