Long noncoding RNA MAGI2-AS3 regulates the H₂O₂ level and cell senescence via HSPA8.

ABSTRACT: The redox homeostasis system regulates many biological processes, intracellular antioxidant production and redox signaling. However, long noncoding RNAs (lncRNAs) involved in redox regulation have rarely been reported. Herein, we reported that downregulation of MAGI2-AS3 decreased the superoxide level in Human fibroblasts (Fbs), a replicative aging model, as detected by the fluorescent probes dihydroethidium (DHE) and MitoSOX™ Red. RNA pulldown combined with mass spectrometry showed that HSPA8 is a novel interacting protein of MAGI2-AS3, which was further confirmed by photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP). Downregulation of MAGI2-AS3 decreased the hydrogen peroxide (H₂O₂) content by stabilizing the HSPA8 protein level

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC9287730 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature