Synaptotagmin 2 is ectopically overexpressed in excitatory presynapses of a widely used CaMKΙΙα-Cre mouse line.

ABSTRACT: The CaMKΙΙα-Cre mouse lines, possibly the most used Cre lines in neuroscience, have resulted in over 800 articles to date. Here, we demonstrate that the second most widely used CaMKΙΙα-Cre line, Tg(Camk2a-cre)2Gsc (or CamiCre), shows ectopic overexpression of synaptotagmin 2, the most efficient Ca²⁺ sensor for fast synchronous neurotransmitter release, in excitatory presynapses of Cre⁺ brains. Moreover, the upregulation of immediate-early genes and genes incorporated in bacterial artificial chromosome (BAC) transgenes, such as L-proline transporter Slc6a7, was found in Cre⁺ hippocampus. The copy number and integration site of the transgene are suggested to have caused the aberrant gene expression in Cre⁺ brains. Most importantly, CamiCre

SUBMITTER: Matsuura K 

PROVIDER: S-EPMC9287804 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature