Both COVID-19 infection and vaccination induce high-affinity cross-clade responses to SARS-CoV-2 variants.

ABSTRACT: The B.1.1.529 (omicron) variant has rapidly supplanted most other SARS-CoV-2 variants. Using microfluidics-based antibody affinity profiling (MAAP), we have characterized affinity and IgG concentration in the plasma of 39 individuals with multiple trajectories of SARS-CoV-2 infection and/or vaccination. Antibody affinity was similar against the wild-type, delta, and omicron variants (K _A ranges: 122 ± 155, 159 ± 148, 211 ± 307 μM^-1, respectively), indicating a surprisingly broad and mature cross-clade immune response. Postinfectious and vaccinated subjects showed different IgG profiles, with IgG3 (p-value = 0.002) against spike being more prominent in the former group. Lastly, we found that the ELISA titers correlated linearly with measured concentrations (R =

SUBMITTER: Emmenegger M 

PROVIDER: S-EPMC9288251 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature