Ontology highlight
ABSTRACT:
SUBMITTER: Jedrzejczyk DJ
PROVIDER: S-EPMC9288538 | biostudies-literature | 2022 Jul
REPOSITORIES: biostudies-literature
Jedrzejczyk D J DJ Poulsen L D LD Mohr M M Damas N D ND Schoffelen S S Barghetti A A Baumgartner R R Weinert B T BT Warnecke T T Gill R T RT
Scientific reports 20220716 1
CRISPR-Cas12a systems are becoming an attractive genome editing tool for cell engineering due to their broader editing capabilities compared to CRISPR-Cas9 counterparts. As opposed to Cas9, the Cas12a endonucleases are characterized by a lack of trans-activating crRNA (tracrRNA), which reduces the complexity of the editing system and simultaneously makes CRISPR RNA (crRNA) engineering a promising approach toward further improving and modulating editing activity of the CRISPR-Cas12a systems. Here ...[more]