Characterization of polymorphic microsatellite markers for the Japanese endangered land snail Mandarina.

ABSTRACT:

Objective

Mandarina is an endangered land snail genus of the oceanic Ogasawara archipelago. On Chichijima Island, the largest inhabited island in Ogasawara, this genus is almost extinct in the wild due to predation by invasive species. Although ex situ conservation programs started in 2010, genetic diversity and population structure remain unclear due to a lack of genetic markers with sufficient genetic variation. In this study, we designed polymorphic microsatellite markers of Mandarina to enable genetic analysis and to develop appropriate conservation plans.

Results

Twenty-three polymorphic microsatellite markers were identified from the genomic DNA of wild samples of Mandarina mandarina. We assessed the genetic diversity of each marker. In 16 markers, neither linkage dise

SUBMITTER: Nishida S 

PROVIDER: S-EPMC9288687 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature