Identification of enhancers responsible for the coordinated expression of myosin heavy chain isoforms in skeletal muscle.

ABSTRACT:

Background

Skeletal muscles consist of fibers of differing contractility and metabolic properties, which are primarily determined by the content of myosin heavy chain (MYH) isoforms (MYH7, MYH2, MYH1, and MYH4). The regulation of Myh genes transcription depends on three-dimensional chromatin conformation interaction, but the mechanistic details remain to be determined.

Results

In this study, we characterized the interaction profiles of Myh genes using 4C-seq (circular chromosome conformation capture coupled to high-throughput sequencing). The interaction profile of Myh genes changed between fast quadriceps and slow soleus muscles. Combining chromatin immunoprecipitation-sequencing (ChIP-seq) and transposase accessible chromatin with high-throughput sequencing (ATAC-seq), we

SUBMITTER: Long K 

PROVIDER: S-EPMC9288694 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature