Psychostimulant-induced aberrant DNA methylation in an in vitro model of human peripheral blood mononuclear cells.

ABSTRACT:

Background

Several reports have provided crucial evidence in animal models that epigenetic modifications, such as DNA methylation, may be involved in psychostimulant-induced stable changes at the cellular level in the brain. Epigenetic editors DNA methyltransferases (DNMTs) and ten-eleven translocation enzymes (TETs) coordinate expression of gene networks, which then manifest as long-term behavioural changes. However, the extent to which aberrant DNA methylation is involved in the mechanisms of substance use disorder in humans is unclear. We previously demonstrated that cocaine modifies gene transcription, via DNA methylation, throughout the brain and in peripheral blood cells in mice.

Results

We treated human peripheral blood mononuclear cells (PBMCs) from healthy male dono

SUBMITTER: Anier K 

PROVIDER: S-EPMC9288712 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature