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Determination of optical density (OD) of oligodeoxynucleotide from HPLC peak area.

ABSTRACT: Oligodeoxynucleotides (ODNs) are typically purified and analysed with HPLC equipped with a UV-Vis detector. Quantities of ODNs are usually determined using a UV-Vis spectrometer separately after HPLC, and are reported as optical density at 260 nm (OD₂₆₀). Here, we describe a method for direct determination of OD₂₆₀ of ODNs using the area of the peaks in HPLC profiles. It is expected that the method will save significant time for researchers in the area of nucleic acid research, and minimize the loss of oligonucleotide samples.

SUBMITTER: Chillar K

PROVIDER: S-EPMC9288849 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Determination of optical density (OD) of oligodeoxynucleotide from HPLC peak area.

Chillar Komal K Yin Yipeng Y Eriyagama Adikari M Dhananjani N AMDN Fang Shiyue S

PeerJ analytical chemistry 20220707

Oligodeoxynucleotides (ODNs) are typically purified and analysed with HPLC equipped with a UV-Vis detector. Quantities of ODNs are usually determined using a UV-Vis spectrometer separately after HPLC, and are reported as optical density at 260 nm (OD<sub>260</sub>). Here, we describe a method for direct determination of OD<sub>260</sub> of ODNs using the area of the peaks in HPLC profiles. It is expected that the method will save significant time for researchers in the area of nucleic acid resea ...[more]

PMID: 35856072

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