Flipped Well-Plate Hanging-Drop Technique for Growing Three-Dimensional Tumors.

ABSTRACT: Three-dimensional (3D) tumor culture techniques are gaining popularity as in vitro models of tumoral tissue analogues. Despite the widespread interest, need, and present-day effort, most of the 3D tumor culturing methodologies have not gone beyond the inventors' laboratories. This, in turn, limits their applicability and standardization. In this study, we introduce a straightforward and user-friendly approach based on standard 96-well plates with basic amenities for growing 3D tumors in a scaffold-free/scaffold-based format. Hanging drop preparation can be easily employed by flipping a universal 96-well plate. The droplets of the medium generated by the well-plate flip (WPF) method can be easily modified to address various mechanisms and processes in cell biology, including cancer.

SUBMITTER: Jeong Y 

PROVIDER: S-EPMC9289396 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature