Trained Immunity in Primary Sjogren's Syndrome: Linking Type I Interferons to a Pro-Atherogenic Phenotype.

ABSTRACT:

Background

Trained immunity - or innate immune memory - can be described as the long-term reprogramming of innate immune cells towards a hyperresponsive state which involves intracellular metabolic changes. Trained immunity has been linked to atherosclerosis. A subgroup of patients with primary Sjögren's syndrome (pSS) exhibits systemic type I interferon (IFN) pathway activation, indicating innate immune hyperactivation. Here, we studied the link between type I IFNs and trained immunity in an in vitro monocytic cell model and peripheral blood mononuclear cells (PBMCs) from pSS patients.

Methods

The training stimuli heat killed Candida albicans, muramyl dipeptide, IFNβ, and patient serum were added to THP-1 cells for 24 hours, after which the cells were washed,

SUBMITTER: Huijser E 

PROVIDER: S-EPMC9289449 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature