Expression of Staphylococcus aureus Virulence Factors in Atopic Dermatitis.

ABSTRACT: Atopic dermatitis (AD) is a skin inflammatory disease in which the opportunistic pathogen Staphylococcus aureus is prevalent and abundant. S. aureus harbors several secreted virulence factors that have well-studied functions in infection models, but it is unclear whether these extracellular microbial factors are relevant in the context of AD. To address this question, we designed a culture-independent method to detect and quantify S. aureus virulence factors expressed at the skin sites. We utilized RNase-H‒dependent multiplex PCR for preamplification of reverse-transcribed RNA extracted from tape strips of patients with AD sampled at skin sites with differing severity and assessed the expression of a panel of S. aureus virulence factors using qPCR. We observed a

SUBMITTER: Poh SE 

PROVIDER: S-EPMC9289736 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature