Induction of reactive oxygen species by mechanical stretch drives endothelin production in neonatal pig renal epithelial cells.

ABSTRACT: Vasoactive endothelin (ET) is generated by ET converting enzyme (ECE)-induced proteolytic processing of pro-molecule big ET to biologically active peptides. H₂O₂ has been shown to increase the expression of ECE1 via transactivation of its promoter. The present study demonstrates that H₂O₂ triggered ECE1-dependent ET1-3 production in neonatal pig proximal tubule (PT) epithelial cells. A uniaxial stretch of PT cells decreased catalase, increased NADPH oxidase (NOX)2 and NOX4, and increased H₂O₂ levels. Stretch also increased cellular ECE1, an effect reversed by EUK-134 (a synthetic superoxide dismutase/catalase mimetic), NOX inhibitor apocynin, and siRNA-mediated knockdown of NOX2 and NOX4. Short-term unilateral ureteral obstr

SUBMITTER: Kumar R 

PROVIDER: S-EPMC9289874 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature